FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb267.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb267.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52814090
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36700.026.17814845
TCTCGTA36950.026.12284344
TCGCGTA10350.023.04321744
TCCGAGC304250.022.6587245
ATCTCGT44550.021.61624143
CGTCTAG36950.019.0022621
CGAGACA69050.017.69348329
TAGGACG135400.017.3319474
ATCGCGT14200.017.2711943
ACGAGAC73500.016.62222728
AATCTCG59550.016.17134542
CTACGGT36100.015.830813
CTCCGAG443450.015.6425144
CTAGACG42100.015.0178153
GGACGTG153450.014.9560396
TCTCCGA468500.014.73911743
TAGACGC59950.014.7122334
CCACGAG84950.014.62017226
TACGGTG39800.014.52864714
CACGAGA85200.014.44524427
Produced by FastQC (version 0.11.5)