FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52796060
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC262100.024.07921445
TAGGACG180500.017.9750754
ACCGTAC20250.017.22201533
GGACGTG189300.016.6403226
GACCGTA21100.016.52823632
CTCCGAG400400.015.79026944
AGGACGT210800.015.6796195
CGTCTAG27400.015.6051061
GACGTGA112300.015.04659757
GTCCTAC256500.014.7922381
TCGCGTA5500.014.31800844
TCTCCGA444850.014.17230443
GTAGGAC235150.013.855033
TATACAC2359350.013.60175745
GTCCTAA198200.013.3185841
TAGACGC41600.012.9266864
CTCGTAT23050.012.78724145
TCTCGTA23200.012.51062644
GACGTGG122200.012.428277
TTAGGAC177850.012.42342953
Produced by FastQC (version 0.11.5)