FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50505906
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC26600.023.76891139
TCCGAGC258850.023.6253245
GGCGAAT30850.020.49442738
TAAGGCG33850.019.27604535
TAGGACG159850.018.368784
CGAATCG9900.018.18190440
CGTCTAG29350.017.481991
CGAATCT28950.016.0881640
GGACGTG178200.016.0480256
AGGCGAA43500.015.77596837
CTCCGAG392700.015.6644144
AGGACGT197500.015.3570045
TCTCCGA414100.014.79539543
AAGGCGA46450.014.77381636
GACGTGA103150.014.3091147
GTCCTAC232300.014.0469791
CGAGACT48350.014.00705529
GTAGGAC216100.013.4104823
TATACAC2186700.013.29587645
ATCTCCG464400.013.16380942
Produced by FastQC (version 0.11.5)