FastQCFastQC Report
Tue 26 Apr 2022
HKKG2BGXL_n01_YZ03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKG2BGXL_n01_YZ03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11997173
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1286881.0726526990983625TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC129270.10775038419467654TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)