FastQCFastQC Report
Sat 26 Mar 2022
HKKF7AFX3_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKF7AFX3_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558190
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG267711.046482083035271No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT244980.9576301994769739No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGAGACGGACGTC198980.7778155649111286No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT181890.7110105191561221No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGTTCTGGTCCTTGTT134900.5273259609333161No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAGCCAGAGATACT115480.4514129130361701No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGGAGACGGACGTCT115010.4495756765525626No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT107590.42057079419433274No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG102110.3991493985982276No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC78490.306818492762461No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCTGGTCGCGGCTGA69590.27202826998776475No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT68920.26940923074517525No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGGTTCTGGTCCTTG68650.2683537970205497No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA65880.2575258288086499No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG64020.25025506315011786No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGG45910.1794628233243035No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAGCCAGAGATAC45110.17633561228837577No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGAGACGGACGTCT44660.1745765560806664No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGTACCGCTGCATGA36500.1426790035142034No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT35940.140489955789054No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAGCCAGAGATACT34890.1363854913043988No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGTTCTGGTCCTTGTT34710.13568186882131505No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCGCTGCATGATC32200.12587024419609177No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA30590.11957673198628718No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG30170.11793494619242512No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGTAGCGCACGATATT29960.11711405329549408No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAGCCAGAGATA29850.11668406177805402No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGTTCTGGTCCTTG29760.11633225053651214No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGCTGAAGGTTCCAGGT29480.11523772667393743No Hit
GATCGCTCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT29110.11379139156982084No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAAACAAGACGGGTGG27030.10566064287640871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)