FastQCFastQC Report
Sat 26 Mar 2022
HKKF7AFX3_n01_Other_eRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKF7AFX3_n01_Other_eRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22099490
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG10953814.956589495956694No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT9464044.28246986695168No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT4818472.1803534832704283No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT4585612.07498453584223No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG4387011.985118208610244No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG2923121.3227092570914531No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA2839961.285079429434797No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG1456450.6590423579910668No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA1394120.6308380872137773No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT1026000.46426410745225344No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGTAGCGCACGATATT859770.3890451770606471No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGCACACAGAGCAGACC831160.3760991769493323No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT825060.37333893225590276No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGCTGAAGGTTCCAGGT778200.35213482302080273No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAATGGAAGAGGGAAGA772780.34968227773582106No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTTGATTTCCAGTGTTT689020.3117809506011225No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAGCCAGAGATACTGGG584420.2644495415957563No Hit
GATCGCTCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT571590.25864397775695275No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA496420.22462961814955912No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGCTCAACCGCGGGGC491490.2223987974383119No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCACTCCTCCTCGTCCCT482330.21825390540686687No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC466000.21086459461281687No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT449710.20349338378396967No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTTCTGGTCCTTGTTCA424050.19188225610636261No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAGCCCTCGTCGCAA399970.18098607705426686No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACTTGGCTTGAGTATCA389080.17605836152779997No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGGCACACAGAGCAGACC371490.16809890182986123No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGTGTTTGTTCCACC337530.1527320313726697No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTCCTCCTCCTCGTCC333750.1510215846610035No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACAGCGGAGACGGACGTCTCTG312820.14155077786862955No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGCGGGACTGCGCGCGG312610.14145575305131475No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGGGCTCCCAGCGGCAA303880.13750543564579998No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGAATGGAAGAGGGAAGA287750.13020662467776406No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGCGGGTCTCCAAAGAT284520.1287450524876366No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAAATGGCGGGTAAG269060.12174941593674786No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCACCCGCACGAGCGAGC255390.1155637528286852No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGAGCCAGAGATACTGGG253010.11448680489911757No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGTTGATTTCCAGTGTTT251290.11370850639539647No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCCTCCTCCGCTCGGCG240510.1088305657732373No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCACAGAGAGCAAATCA237110.10729206873099786No Hit
GATCGCACGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT234930.10630562062744434No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT234130.105943621323388No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGGCAGATGAACTTCAC231480.10474449862870139No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGGGCCTCCCTGCTGA228210.1032648264733711No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAAGGAGGCGAACCC227450.10292092713451759No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)