FastQCFastQC Report
Sat 26 Mar 2022
HKKF7AFX3_n01_K562_eRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKF7AFX3_n01_K562_eRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24998392
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGTTCTGGTCCTTGTT5979502.3919538504716624No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGAGACGGACGTC3180261.272185826992392No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGGTTCTGGTCCTTG3076431.2306511554823205No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC2400370.9602097606918077No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGGAGACGGACGTCT2205230.8821487398069443No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAGCCAGAGATAC2052660.8211168142334915No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG2030770.812360251011345No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT2025970.8104401275090014No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGTTCTGGTCCTTGTT1579730.6319326459077848No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCGCTGCATGATC1498590.5994745582035836No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAGCCAGAGATA1459730.583929558349193No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGTTCTGGTCCTTG1280230.5121249398761328No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAGCCAGAGATA1142640.4570853997329108No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT1067380.42697946331908065No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGAGACGGACGTCT980130.3920772184066879No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT901610.36066719811418274No Hit
GATCGCTCGGTTCTTGTGGAAAGGACGAAACTCCGGTTCTGGTCCTTGTT865850.34636227802172237No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT842880.3371736870115486No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGG837220.33490954138170165No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGGTTCTGGTCCTTGTT821390.3285771340812641No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGCTGAAGGTTCCAG750930.3003913211697776No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT700220.28010601641897603No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG694110.27766185921078446No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT655220.26210485858450416No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGTACCGCTGCATGA652570.2610447904009186No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGCTAAGCTTGGAA587220.23490310896796882No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGAGGAATGGAAA550160.22007815542695705No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT524030.2096254831110737No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGTGGCTGGGCGACAA482880.19316442433577327No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATAT473310.1893361781029756No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGATTGGCCAGAAA438750.17551128888610115No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT437270.17491925080621185No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC430890.17236708665101338No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGTTCTGGTCCTTG416450.1665907151147962No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTCACAGGCCATT402420.1609783541277375No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA385050.15402990720363133No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCGCTGCATGATC384950.15398990463066586No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGGTTCTGGTCCTTG380980.15240180248393576No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC373000.14920959716128943No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGTTCTCAGAAAT361380.1445612981826991No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGTAGCGCACGAT325690.1302843798913146No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTGTTTTGCATGT319170.12767621213396446No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGCTGAAGGTTCCAG317360.12695216556328903No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCGGCAGCGGCGGTTC316360.12655213983363411No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGATTCCTGGGAA316040.1264241316001445No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGGAACCAGAGGA315290.12612411230290332No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGAAAGAGAAATA314480.12580009146188284No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCAAGGGTGCCATCC301850.12074776649634104No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA281730.11269924881568381No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGCTGAAGGTTCCA280800.11232722488710473No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAGCCAGAGATAC277570.11103514178031931No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAGGGTGTGGCCAAA261280.10451872264424047No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCGCTGCATGA257220.10289461818184145No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCAGATCATAGGTAT256740.10270260583160708No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGGTAGCGCACGATA250720.10029445093908439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)