FastQCFastQC Report
Sat 26 Mar 2022
HKKF7AFX3_n01_A375_eRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKF7AFX3_n01_A375_eRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19104529
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGAGACGGACGTC4504772.3579592043331714No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAGCCAGAGATACT3874692.028152591461428No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCTGGTCGCGGCTGA2365311.2380886228600558No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT2354361.2323569976522322No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGGAGACGGACGTCT2288051.197647950389146No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAGCCAGAGATACT1188640.6221770764408796No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT1185560.6205648932774003No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGG967310.5063249661899542No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAAACAAGACGGGTGG863810.45214933066394886No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC848970.4443815390580945No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGGAGACGGACGTCT791080.4140798236899742No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGTACCGCTGCATGA733870.38413404486444025No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGACAGTTGACTTC583700.3055296469229888No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAGCCAGAGATACT574970.30096004983949093No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC496060.25965570781671715No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGTAGCGCACGAT492550.2578184471336613No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGCTGAAGGTTCCA430040.22509845702032225No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGACCTGCCCTAGC428800.22444939626619426No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT416150.21782792970190473No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATAT406050.21254122517231386No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT404260.21160427456756456No Hit
GATCGCTCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT386720.2024232055132058No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAGCCAGAGATACT374370.19595876977652787No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGTAGCGCACGATATT357650.1872069183176408No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGCTGAAGGTTCCAGGT351690.18408723920909015No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT334810.1752516379754769No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG333690.17466538955239358No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGGCTCCATGTGTG308470.16146433131117757No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGCTTCTCTCTCTC304080.1591664468671277No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGTAGGCGCGCCGCTC303750.15899371295675493No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGGTAGCGCACGATA282430.14783405547448986No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT281220.147200697803123No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGCCCCTCAGGCCC266740.13962134319040265No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCGCTGCATGA250970.13136675601895237No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGTTCTGGTCCTTGTTC240800.1260434109629188No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACAAGACGGGTGG236340.12370888599242619No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGACTGCTGCTGTC233480.1222118587691955No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCATAACCCCTGAAG233000.12196060944501692No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGCTGAAGGTTCCAG232370.12163084470703256No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGCGCCGTTCCGAG200430.1049122959273165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)