FastQCFastQC Report
Tue 23 May 2017
HKJ5TBGX2_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKJ5TBGX2_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171733815
Sequences flagged as poor quality0
Sequence length32
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18846401.0974192822770519No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTA2432650.1416523589137061No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCAT2278320.13266577697583903No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTC1803110.10499446483501226No Hit
GTGTACAAGCTCGTAACGAAGGGCGCGATCTT1768280.1029663261134681No Hit
ACTATATGTTGTTGGGTGAGGCATATGTGAAT1741560.10141042985622836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGC482650.017.62902826
GCTTCCG567900.014.8887325
GGCTTCA545350.014.47425414
TCAGGGG561000.014.46203818
CTCCGAA151600.014.4602526
ACCGGTG598150.014.0404368
ATACCGG617200.014.0224426
GGTATTC527450.013.99817324
GTTATAC648000.013.837093
GGGTATT547700.013.60874223
GGACCAT312250.013.5089446
TTCAGGG589150.013.43152717
TATACCG715350.013.3723855
GGGGTAT567650.013.23764622
CGTTCTA129850.013.22746625
TGGCTTC600800.013.2098213
CCGGTGG621150.013.18701659
GGGGGTA623800.012.9044821
TTATACC703400.012.7301384
GCTTCAG639250.012.62057615
Produced by FastQC (version 0.11.2)