FastQCFastQC Report
Tue 23 May 2017
HKJ5TBGX2_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKJ5TBGX2_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171733815
Sequences flagged as poor quality0
Sequence length26
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAA2278750.019.7702481
TTCCAGA4003250.019.7477251
TCGACCA1929750.019.7406122
AACTACC2104550.019.7308836
TACTCCA2031400.019.7255923
TCAGAAC2114400.019.7131982
TCCACAC2140500.019.7117421
TCCTTAC2178400.019.7115634
CACCTTC2389700.019.6998963
CACAGAA3969350.019.6974161
CCACACT2157050.019.691532
TTCCACC1308700.019.6882252
GCCGAAC2152500.019.6825245
TACAAGA2282850.019.680123
CCGTGAC2483550.019.6758254
CTCCTAC1436600.019.6673374
TCGCATC1754400.019.6667464
CCTTACG2182950.019.6645165
CTACTCC2040700.019.6562562
TTCGACC1929200.019.6518121
Produced by FastQC (version 0.11.2)