FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n02_wt-37d-30ar-1.35200000093fef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n02_wt-37d-30ar-1.35200000093fef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28030124
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2020870.720963631841229Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT293750.10479796664474264Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG428050.036.7365945
GGTCGCC428950.036.19138744
GTGGTCG430050.034.29760742
TGGTCGC436850.033.7850643
CTCGGTG452800.032.86902238
ATCTCGG477700.032.18005436
TCTCGGT467900.031.7510837
GGTGGTC464700.031.69511641
GATCTCG500600.030.73565935
TCGGTGG489700.030.53947839
CGGTGGT487500.030.34989240
TCGCCGT80950.028.7757245
GCACACG51100.028.66213618
AGATCTC540550.028.56036834
TAGATCT590250.027.07735633
GTAGATC623250.026.76409132
AGTGTAG574250.026.64112729
GTGTAGA639000.026.03749830
GAGTGTA582750.025.57245328
TGTAGAT665050.024.94654731
Produced by FastQC (version 0.11.2)