HKHNTBCXY_l02n02_k-asyn-2.35200000093f18.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKHNTBCXY_l02n02_k-asyn-2.35200000093f18.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17788559
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	1574150	8.849227191477398	Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	147499	0.8291790245629227	Illumina Single End PCR Primer 1 (100% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	57018	0.32053186545351986	Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	44064	0.2477097779533463	Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	31824	0.178901506299639	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	23938	0.13456964108222594	Illumina Single End PCR Primer 1 (100% over 39bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	30775	41.867504	18
GAGCACA	48690	41.207325	16
AGAGCAC	48910	41.19642	15
AGCACAC	36240	40.495537	17
CACACGA	17120	40.06645	19
ACACGAG	17125	40.041615	20
TCGCCGT	23205	39.86227	45
GTCGCCG	211180	39.82224	45
GGTCGCC	214430	39.197407	44
GTGGTCG	215915	38.877846	42
TGGTCGC	215980	38.82649	43
GGTGGTC	217970	38.503056	41
CACGAGA	17825	38.47034	21
CGGTGGT	219525	38.211174	40
AAGAGCA	60135	38.017963	14
CACACGT	15275	37.968975	19
GGTCGGA	7235	37.462883	2
CTCGGTG	225025	37.240494	38
TCGGTGG	226735	36.989613	39
TCTCGGT	227115	36.80408	37