FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n02_k-asyn-1.35200000093efd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n02_k-asyn-1.35200000093efd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16284157
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG61750.025.84155545
GGTCGCC58350.025.57207944
GGCGGGG33650.021.993251
CTCGGTG62250.021.97838
ATCTCGG71050.021.82303236
GGCGGGC21400.021.338351
CGGGGAC17800.020.7253761
TCTCGGT69950.019.78442237
GATCTCG81100.019.4242335
TGGTCGC68200.018.71072843
GCGGGTG38300.018.6769921
GTGGTCG69200.018.30974642
GGCGGGA47300.018.2620141
GTAGATC114000.017.74805832
GGGGACC23000.017.505392
GGCGGTG57900.017.1332041
GCGGGAA50650.017.0985721
GGGCGTC21850.016.8838311
GGGCGAC32550.016.8623221
AGATCTC96250.016.85822534
Produced by FastQC (version 0.11.2)