Basic Statistics
Measure | Value |
---|---|
Filename | HKHNTBCXY_l02n02_k-30ar-2.35200000093ed3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7503 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 93 | 1.2395041983206718 | Illumina Single End PCR Primer 1 (100% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACACGTC | 20 | 6.5941014E-4 | 45.000004 | 20 |
GCACACG | 35 | 1.0108852E-7 | 45.000004 | 18 |
CACACGT | 25 | 3.5316734E-5 | 45.0 | 19 |
AAGAGCA | 35 | 5.4596894E-6 | 38.57143 | 14 |
GAGCACA | 35 | 5.4596894E-6 | 38.57143 | 16 |
AGAGCAC | 35 | 5.4596894E-6 | 38.57143 | 15 |
AGCACAC | 35 | 5.4596894E-6 | 38.57143 | 17 |
GTGGTCG | 35 | 5.4596894E-6 | 38.57143 | 42 |
TCGGTGG | 30 | 1.0354707E-4 | 37.499996 | 39 |
TCTCGGT | 30 | 1.0354707E-4 | 37.499996 | 37 |
CGGTGGT | 30 | 1.0354707E-4 | 37.499996 | 40 |
CTCGGTG | 30 | 1.0354707E-4 | 37.499996 | 38 |
GGTGGTC | 30 | 1.0354707E-4 | 37.499996 | 41 |
GAGTGTA | 40 | 1.3648067E-5 | 33.750004 | 28 |
GTCGCCG | 40 | 1.3648067E-5 | 33.750004 | 45 |
AGATCTC | 40 | 1.3648067E-5 | 33.750004 | 34 |
TGGTCGC | 35 | 2.5638362E-4 | 32.14286 | 43 |
GTGTAGA | 35 | 2.5638362E-4 | 32.14286 | 30 |
AAGAGTG | 35 | 2.5638362E-4 | 32.14286 | 26 |
GATCTCG | 35 | 2.5638362E-4 | 32.14286 | 35 |