FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n02_k-30ar-2.35200000093ed3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n02_k-30ar-2.35200000093ed3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7503
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG931.2395041983206718Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC206.5941014E-445.00000420
GCACACG351.0108852E-745.00000418
CACACGT253.5316734E-545.019
AAGAGCA355.4596894E-638.5714314
GAGCACA355.4596894E-638.5714316
AGAGCAC355.4596894E-638.5714315
AGCACAC355.4596894E-638.5714317
GTGGTCG355.4596894E-638.5714342
TCGGTGG301.0354707E-437.49999639
TCTCGGT301.0354707E-437.49999637
CGGTGGT301.0354707E-437.49999640
CTCGGTG301.0354707E-437.49999638
GGTGGTC301.0354707E-437.49999641
GAGTGTA401.3648067E-533.75000428
GTCGCCG401.3648067E-533.75000445
AGATCTC401.3648067E-533.75000434
TGGTCGC352.5638362E-432.1428643
GTGTAGA352.5638362E-432.1428630
AAGAGTG352.5638362E-432.1428626
GATCTCG352.5638362E-432.1428635