Basic Statistics
Measure | Value |
---|---|
Filename | HKHNTBCXY_l02n02_hk-37d-30ar-2.35200000093fb8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24619743 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1707643 | 6.936071590999142 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 206665 | 0.8394279339146635 | Illumina Single End PCR Primer 1 (100% over 51bp) |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 89403 | 0.36313539097463365 | Illumina Single End PCR Primer 1 (98% over 51bp) |
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 68106 | 0.2766316447738711 | Illumina Single End PCR Primer 1 (98% over 51bp) |
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 56438 | 0.22923878612380316 | Illumina Single End PCR Primer 1 (98% over 51bp) |
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 35124 | 0.14266598964903898 | Illumina Single End PCR Primer 1 (100% over 47bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 70445 | 0.0 | 42.523712 | 18 |
GAGCACA | 109635 | 0.0 | 42.39556 | 16 |
AGAGCAC | 110260 | 0.0 | 42.346527 | 15 |
AGCACAC | 81935 | 0.0 | 41.991695 | 17 |
CACACGA | 37655 | 0.0 | 41.91701 | 19 |
ACACGAG | 37945 | 0.0 | 41.58454 | 20 |
CACGAGA | 38960 | 0.0 | 40.508507 | 21 |
CACACGT | 34050 | 0.0 | 40.375565 | 19 |
AAGAGCA | 131415 | 0.0 | 40.165012 | 14 |
TCGCCGT | 33045 | 0.0 | 39.73489 | 45 |
GTCGCCG | 258030 | 0.0 | 38.98087 | 45 |
GGTCGCC | 260800 | 0.0 | 38.549038 | 44 |
GTGGTCG | 262325 | 0.0 | 38.246635 | 42 |
GGTCGGA | 11435 | 0.0 | 38.10125 | 2 |
ACACGTC | 19690 | 0.0 | 38.0926 | 20 |
TGGTCGC | 264460 | 0.0 | 37.894695 | 43 |
CACGTAG | 16065 | 0.0 | 37.839214 | 21 |
GGTGGTC | 266555 | 0.0 | 37.634865 | 41 |
ACACGTA | 16435 | 0.0 | 37.41025 | 20 |
CGGTGGT | 269300 | 0.0 | 37.220036 | 40 |