FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n02_hk-37d-30ar-2.35200000093fb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n02_hk-37d-30ar-2.35200000093fb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24619743
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG17076436.936071590999142Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2066650.8394279339146635Illumina Single End PCR Primer 1 (100% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG894030.36313539097463365Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG681060.2766316447738711Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG564380.22923878612380316Illumina Single End PCR Primer 1 (98% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT351240.14266598964903898Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG704450.042.52371218
GAGCACA1096350.042.3955616
AGAGCAC1102600.042.34652715
AGCACAC819350.041.99169517
CACACGA376550.041.9170119
ACACGAG379450.041.5845420
CACGAGA389600.040.50850721
CACACGT340500.040.37556519
AAGAGCA1314150.040.16501214
TCGCCGT330450.039.7348945
GTCGCCG2580300.038.9808745
GGTCGCC2608000.038.54903844
GTGGTCG2623250.038.24663542
GGTCGGA114350.038.101252
ACACGTC196900.038.092620
TGGTCGC2644600.037.89469543
CACGTAG160650.037.83921421
GGTGGTC2665550.037.63486541
ACACGTA164350.037.4102520
CGGTGGT2693000.037.22003640