HKHNTBCXY_l02n02_hk-37d-30ar-1.35200000093f9f.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKHNTBCXY_l02n02_hk-37d-30ar-1.35200000093f9f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4549911
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	399155	8.772808962636852	Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	33248	0.7307395683124351	Illumina Single End PCR Primer 1 (100% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	18419	0.40482110529194965	Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	13622	0.29939047159383997	Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	11729	0.2577852621732601	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	6607	0.14521163161213482	Illumina Single End PCR Primer 1 (100% over 47bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	5744	0.12624422763434273	Illumina Single End PCR Primer 1 (100% over 39bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	21280	43.26314	18
GAGCACA	32460	43.188023	16
AGAGCAC	32725	43.064224	15
AGCACAC	24345	42.982403	17
CACACGA	11590	42.724438	19
ACACGAG	11645	42.502865	20
CACACGT	9755	42.181908	19
CACGAGA	11855	41.69349	21
AAGAGCA	38275	41.438507	14
GCACAAG	5895	40.569862	18
GTCGCCG	56860	40.507984	45
ACACGTC	5470	40.10073	20
CACGTAG	4675	40.038837	21
TCGCCGT	5405	40.01564	45
ACACGTA	4690	40.006283	20
GGTCGCC	57555	39.968	44
GTGGTCG	57915	39.569717	42
TGGTCGC	58195	39.318752	43
AGCACAA	6120	39.298897	17
ACGAGAT	12515	39.225063	22