FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n02_h-asyn-2.35200000093f4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n02_h-asyn-2.35200000093f4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15662406
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG917940.5860785373588197Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT723090.46167236374794524Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT186620.11915155308833139Illumina Single End PCR Primer 1 (100% over 47bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT129450.037.34499745
CTCGGGG20600.031.45961438
TCTCGGG24250.028.3022137
GGGTCGC32750.027.76174243
GATCGCG24150.026.74381635
ATCGCGG24800.026.58672536
TCGCCGG14900.026.2822945
GTCGCCG327450.026.27601645
GGTCGCC320800.025.86542544
GCGGCGC37900.024.8687821
GCGGCCG29000.024.67910245
GGCCGCC28450.023.49389544
TGGTCGC309750.023.40943343
ATCTCGG366650.023.19391836
CTCGTCG22050.023.05738411
GTGGTCG314750.023.00136842
CTCGGTG339200.022.99990838
GCCGCCG30750.022.90864845
CGGGGAC21100.022.7078931
TCTCGGT347900.022.56725937
Produced by FastQC (version 0.11.2)