FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n02_h-asyn-1.35200000093f32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n02_h-asyn-1.35200000093f32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13324063
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1762531.322817221743848Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT148960.11179773016684175Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG249600.039.28658345
GGTCGCC253900.038.63765344
GCACACG65500.038.3328318
GTGGTCG263450.037.21926542
TGGTCGC267650.036.55154443
GAGCACA96800.035.76936716
GGTGGTC276050.035.53460341
AGCACAC79100.035.49654417
AGAGCAC98900.035.30518715
CTCGGTG279200.034.8414438
CGGTGGT283250.034.6140240
TCTCGGT289650.033.56137537
ATCTCGG292100.033.37382536
TCGGTGG294050.033.19655239
CACACGT46300.033.1390419
GATCTCG309100.031.51765835
ACACGTC35750.030.52147720
CACACGA31450.030.40219119
AGATCTC328550.029.68685534
TCGCCGT33550.029.58334745
Produced by FastQC (version 0.11.2)