FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n02_h-37d-30ar-2.35200000094014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n02_h-37d-30ar-2.35200000094014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9770558
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2114362.164011512955555Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT315850.32326710511313683Illumina Single End PCR Primer 1 (100% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG137850.14108713135933484Illumina Single End PCR Primer 1 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG328050.037.49466345
GGTCGCC330300.037.16277344
GTGGTCG336350.036.37944842
TCGCCGT53900.036.2869445
GCACACG32600.036.23179618
TGGTCGC341550.035.78696443
GGTGGTC348400.035.10540841
CTCGGTG348450.034.85424838
ATCTCGG354250.034.27881636
TCTCGGT353500.034.25482637
CGGTGGT359350.033.95253840
TCGGTGG364600.033.35323339
AGAGCAC54250.033.2180615
GAGCACA54500.032.81833316
GATCTCG377450.032.13707735
GGTCGGA20750.031.5438082
AGCACAC42700.031.45535517
AGATCTC401450.030.27857234
CACACGT21450.029.26207219
TAGATCT435750.028.04913733
Produced by FastQC (version 0.11.2)