FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_wt-37d-30ar-1.35100000093fe2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_wt-37d-30ar-1.35100000093fe2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28030124
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC2585240.922307728642228TruSeq Adapter, Index 14 (98% over 50bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG361360.1289184450272143Illumina Paired End PCR Primer 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC304650.10868664013045394TruSeq Adapter, Index 14 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG366000.033.56749740
CGTATGC391350.033.11726845
GAGCGGT132600.033.0187210
CTCGTAT398000.032.31540743
AGAGCGG137700.031.9101779
ATCTCGT390250.031.72379341
TCGTATG408950.031.68099244
TCTCGTA404750.030.66463742
GCGGTTC144900.030.13825412
AAGAGCG153100.028.8914438
TGCCGAG91200.028.79066327
GCCGAGA89850.028.57252528
AGCGGTT157800.027.8740911
CCGAGAG64900.027.38819729
AATGCCG113850.027.13376425
ATGCCGA100600.027.10692626
GGAATGC142500.027.06234423
GTTCCAT458350.026.72074136
CGGTTCA165100.026.61436513
GTCACAG521050.026.60507630
Produced by FastQC (version 0.11.2)