Basic Statistics
Measure | Value |
---|---|
Filename | HKHNTBCXY_l02n01_k-asyn-2.35100000093f1b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17788559 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC | 1685348 | 9.474336847633358 | TruSeq Adapter, Index 7 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC | 154607 | 0.8691372921213012 | TruSeq Adapter, Index 7 (100% over 51bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC | 30410 | 0.1709525768782058 | TruSeq Adapter, Index 7 (98% over 50bp) |
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT | 24199 | 0.13603687628660646 | TruSeq Adapter, Index 7 (100% over 38bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC | 19206 | 0.10796827331544954 | TruSeq Adapter, Index 7 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 215280 | 0.0 | 38.809273 | 45 |
CATCTCG | 209325 | 0.0 | 37.979424 | 40 |
CTCGTAT | 218630 | 0.0 | 37.96299 | 43 |
TCGTATG | 220270 | 0.0 | 37.87901 | 44 |
ATCTCGT | 211590 | 0.0 | 37.749393 | 41 |
TCTCGTA | 211945 | 0.0 | 37.710163 | 42 |
TCATCTC | 220535 | 0.0 | 35.956043 | 39 |
GATCATC | 221710 | 0.0 | 35.683277 | 37 |
ATCATCT | 224090 | 0.0 | 35.386635 | 38 |
GTATGCC | 29455 | 0.0 | 35.15524 | 45 |
AGATCAT | 228235 | 0.0 | 34.818512 | 36 |
CAGATCA | 247145 | 0.0 | 33.404465 | 35 |
AGTCACC | 269615 | 0.0 | 31.010084 | 29 |
TCACCAG | 269030 | 0.0 | 30.942148 | 31 |
ACCAGAT | 268640 | 0.0 | 30.897448 | 33 |
CACCAGA | 269635 | 0.0 | 30.877728 | 32 |
GTCACCA | 271290 | 0.0 | 30.773956 | 30 |
CCAGATC | 270770 | 0.0 | 30.539717 | 34 |
CAGTCAC | 278595 | 0.0 | 30.215666 | 28 |
CCAGTCA | 283115 | 0.0 | 29.722519 | 27 |