FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_k-asyn-2.35100000093f1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_k-asyn-2.35100000093f1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17788559
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC16853489.474336847633358TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1546070.8691372921213012TruSeq Adapter, Index 7 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC304100.1709525768782058TruSeq Adapter, Index 7 (98% over 50bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT241990.13603687628660646TruSeq Adapter, Index 7 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC192060.10796827331544954TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2152800.038.80927345
CATCTCG2093250.037.97942440
CTCGTAT2186300.037.9629943
TCGTATG2202700.037.8790144
ATCTCGT2115900.037.74939341
TCTCGTA2119450.037.71016342
TCATCTC2205350.035.95604339
GATCATC2217100.035.68327737
ATCATCT2240900.035.38663538
GTATGCC294550.035.1552445
AGATCAT2282350.034.81851236
CAGATCA2471450.033.40446535
AGTCACC2696150.031.01008429
TCACCAG2690300.030.94214831
ACCAGAT2686400.030.89744833
CACCAGA2696350.030.87772832
GTCACCA2712900.030.77395630
CCAGATC2707700.030.53971734
CAGTCAC2785950.030.21566628
CCAGTCA2831150.029.72251927