FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_k-asyn-1.35100000093ef0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_k-asyn-1.35100000093ef0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16284157
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC167200.10267648488036561TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT59950.029.72314310
AGAGCGG63150.028.216989
GCGGTTC66900.026.39989712
AAGAGCG73150.024.7286518
ATGCCGA46800.024.1343326
AGCGGTT74050.024.00273711
TGCCGAG46100.023.6222827
GCCGAGA45000.023.50037228
AATGCCG57550.022.75378825
CAGCAGG79350.022.17269718
CCGAGAG32850.021.91815629
GGAATGC69300.021.81772223
CGGTTCA82500.021.54427513
GGTTCAG84100.021.29490914
AGCAGGA83400.021.01503619
AGGAATG79500.019.64079522
GAATGCC73950.019.47232624
CGAGAGA39500.018.912130
GCAGGAA94350.018.28993620
GTTCAGC98450.018.23668515
Produced by FastQC (version 0.11.2)