FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_k-37d-30ar-1.35100000093f78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_k-37d-30ar-1.35100000093f78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC451.0660980810234542TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC110.2606017531390666TruSeq Adapter, Index 2 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC80.18952854773750297TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)