FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_k-30ar-2.35100000093ed6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_k-30ar-2.35100000093ed6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7503
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC2022.692256430761029TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC170.2265760362521658TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACAC80.10662401705984273TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAC355.4596894E-638.5714328
CCAGTCA355.4596894E-638.5714327
ACTCCAG501.657967E-631.524
CTCCAGT453.0556228E-530.025
TCCAGTC453.0556228E-530.026
AGTCACA300.00483162829.99999829
AACTCCA553.4882305E-628.63636423
CTGAACT553.4882305E-628.63636420
GAACTCC553.4882305E-628.63636422
ACGTCTG606.867558E-626.24999816
GTCTGAA606.867558E-626.24999818
TCTGAAC606.867558E-626.24999819
TGAACTC606.867558E-626.24999821
CGTCTGA606.867558E-626.24999817
CACGTCT651.2787594E-524.23076815
ACACGTC702.2706836E-522.50000214
CACACGT806.366137E-519.68750213
GCACACG851.0148479E-418.52941112
AGCACAC1003.521558E-415.7511
AGATCGG1003.521558E-415.751
Produced by FastQC (version 0.11.2)