FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_hk-37d-30ar-2.35100000093fbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_hk-37d-30ar-2.35100000093fbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24619743
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC20049408.143626844520675TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC2260160.9180274546326498TruSeq Adapter, Index 8 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGC447150.18162252952843577TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC275610.11194674127995567TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2605550.038.14241445
CTCGTAT2620950.037.6094743
TCGTATG2646050.037.5220544
ATCTCGT2534850.037.33640341
TCTCGTA2544300.037.20988542
AATCTCG2533050.037.18705440
GTATGCC387050.036.6769645
GAATCTC2686450.034.96059839
TGAATCT2708950.034.69015538
TTGAATC2728200.034.4206437
CTTGAAT2806750.033.6753336
ACTTGAA3046350.032.345935
CACTTGA3102100.031.7755534
ACACTTG3238350.030.49768833
GTCACAC3287200.030.19711730
CACACTT3304550.030.01950532
TCACACT3306000.029.9852431
AGTCACA3434650.029.35217929
CAGTCAC3472250.029.1574528
CCAGTCA3566250.028.39653827
Produced by FastQC (version 0.11.2)