FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_hk-37d-30ar-1.35100000093f92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_hk-37d-30ar-1.35100000093f92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4549911
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC4530359.957007950265401TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC361920.7954441306654131TruSeq Adapter, Index 6 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC81900.18000352094799216TruSeq Adapter, Index 6 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC61970.13620046633879213TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA59640.1310794870493071TruSeq Adapter, Index 6 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC48390.10635372867733017TruSeq Adapter, Index 6 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC574450.039.08801745
CTCGTAT581050.038.46588543
TCGTATG582350.038.46502344
TATCTCG560000.038.30095740
TCTCGTA566000.038.17280642
ATCTCGT566450.038.13495641
ATATCTC620550.034.5528739
GTATGCC73600.034.11891645
AATATCT629500.034.02586738
CAATATC632950.033.81907337
CCAATAT637900.033.62013236
GCCAATA676750.032.80064835
CGCCAAT688300.032.35016334
CACGCCA702700.031.8185132
GTCACGC707700.031.77811230
TCACGCC705800.031.7680231
ACGCCAA704150.031.72422833
AGTCACG715450.031.52788429
CAGTCAC748400.030.6388728
CCAGTCA770350.029.81193527