Basic Statistics
Measure | Value |
---|---|
Filename | HKHNTBCXY_l02n01_hk-37d-30ar-1.35100000093f92.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 4549911 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 453035 | 9.957007950265401 | TruSeq Adapter, Index 6 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC | 36192 | 0.7954441306654131 | TruSeq Adapter, Index 6 (100% over 51bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC | 8190 | 0.18000352094799216 | TruSeq Adapter, Index 6 (98% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC | 6197 | 0.13620046633879213 | TruSeq Adapter, Index 14 (97% over 38bp) |
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA | 5964 | 0.1310794870493071 | TruSeq Adapter, Index 6 (100% over 38bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC | 4839 | 0.10635372867733017 | TruSeq Adapter, Index 6 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 57445 | 0.0 | 39.088017 | 45 |
CTCGTAT | 58105 | 0.0 | 38.465885 | 43 |
TCGTATG | 58235 | 0.0 | 38.465023 | 44 |
TATCTCG | 56000 | 0.0 | 38.300957 | 40 |
TCTCGTA | 56600 | 0.0 | 38.172806 | 42 |
ATCTCGT | 56645 | 0.0 | 38.134956 | 41 |
ATATCTC | 62055 | 0.0 | 34.55287 | 39 |
GTATGCC | 7360 | 0.0 | 34.118916 | 45 |
AATATCT | 62950 | 0.0 | 34.025867 | 38 |
CAATATC | 63295 | 0.0 | 33.819073 | 37 |
CCAATAT | 63790 | 0.0 | 33.620132 | 36 |
GCCAATA | 67675 | 0.0 | 32.800648 | 35 |
CGCCAAT | 68830 | 0.0 | 32.350163 | 34 |
CACGCCA | 70270 | 0.0 | 31.81851 | 32 |
GTCACGC | 70770 | 0.0 | 31.778112 | 30 |
TCACGCC | 70580 | 0.0 | 31.76802 | 31 |
ACGCCAA | 70415 | 0.0 | 31.724228 | 33 |
AGTCACG | 71545 | 0.0 | 31.527884 | 29 |
CAGTCAC | 74840 | 0.0 | 30.63887 | 28 |
CCAGTCA | 77035 | 0.0 | 29.811935 | 27 |