FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_h-asyn-2.35100000093f42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_h-asyn-2.35100000093f42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15662406
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC1258060.8032354671434261TruSeq Adapter, Index 21 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC795970.5082041673546197TruSeq Adapter, Index 21 (98% over 51bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG474000.30263549546602225Illumina Paired End PCR Primer 2 (97% over 34bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC282800.18055974286453816Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT201650.033.96318410
AGAGCGG204400.033.605319
GTATGCC126850.033.11717245
GCGGTTC207550.032.94351612
AAGAGCG210650.032.7043658
TGCCGAG118750.032.66476427
ATGCCGA123650.032.51668526
AATGCCG148700.032.10767725
GCCGAGA117450.032.05003428
GGAATGC183150.031.76814823
AGCGGTT215900.031.72152111
CCGAGAG76300.031.553329
CGGTTCA217450.031.52644713
CAGCAGG217750.030.8940618
GGTTCAG222150.030.81893514
AGCAGGA220800.030.20237719
GTTCAGC227050.030.11419115
GAATGCC181250.030.04082124
AGGAATG202250.029.68006722
CGAGAGA83750.029.36485530
Produced by FastQC (version 0.11.2)