FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_h-asyn-1.35100000093f35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_h-asyn-1.35100000093f35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13324063
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1883901.4139080549228866TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC154250.11576799058965721TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC265150.035.8282545
CTCGTAT270050.034.9534543
AATCTCG264600.034.50889240
ATCTCGT266700.034.32153741
TCGTATG277300.034.2827744
TCTCGTA275600.033.31882542
TGTAATC288600.031.35065337
GTAATCT289950.031.24348638
TAATCTC300100.030.33672539
CCTTGTA333050.028.14527934
AGTCACC346600.027.45333929
TTGTAAT332750.027.37345736
GTCACCT348050.027.22321130
CTTGTAA349300.026.80441535
CAGTCAC361900.026.69059828
ACCTTGT353900.026.60790433
TCCAGTC369500.026.27528826
CCAGTCA370900.026.2367727
CACCTTG362250.026.06911832
CTCCAGT388750.025.03179425
Produced by FastQC (version 0.11.2)