FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_h-37d-30ar-2.35100000094017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_h-37d-30ar-2.35100000094017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9770558
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC2334362.3891777726512653TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC325710.33335864747949917TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC336900.035.87190245
CTCGTAT338950.035.3298443
TCGTATG343150.035.21198744
ATCTCGT320950.035.1248141
GATCTCG318900.035.02602440
GGATCTC324650.034.31556339
TCTCGTA330550.034.21273442
GTGGATC326800.033.9245537
TGGATCT337650.032.987738
GTATGCC66150.032.4162945
AGTGGAT345950.032.254836
GAGTGGA373500.031.69503835
CGAGTGG389550.030.48033134
ACGAGTG411950.029.05235333
CACGAGT418500.028.65679232
TCACGAG427250.028.16996631
GTCACGA428350.028.11863930
AGTCACG431850.027.98338529
CAGTCAC459700.026.74814628
CCAGTCA465550.026.4069327
Produced by FastQC (version 0.11.2)