FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l02n01_h-37d-30ar-1.3510000009400a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l02n01_h-37d-30ar-1.3510000009400a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67057
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG351.1892007E-745.02
TGGAGTT351.1892007E-745.06
AGACGTG351.1892007E-745.014
TCAGACG351.1892007E-745.012
GTGACTG351.1892007E-745.01
CAGACGT351.1892007E-745.013
AGTTCAG351.1892007E-745.09
GAGTTCA403.393725E-739.3750048
GACGTGT403.393725E-739.37500415
CGTGTGC403.393725E-739.37500417
TCCGATC403.393725E-739.37500427
ACTGGAG403.393725E-739.3750044
TCTTCCG403.393725E-739.37500424
GTGTGCT403.393725E-739.37500418
CCGATCT403.393725E-739.37500428
GGAGTTC403.393725E-739.3750047
ACGTGTG403.393725E-739.37500416
GTGCTCT403.393725E-739.37500420
CGATCTT250.002093018336.00000429
TTCCGAT458.538609E-735.026
Produced by FastQC (version 0.11.2)