FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_k-30ar-1.35200000094021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_k-30ar-1.35200000094021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14192883
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG185170.13046679804237094Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG96000.034.67337845
GGTCGCC93400.034.57822444
CTCGGTG95150.031.68940438
ATCTCGG103900.030.81962236
TCTCGGT99000.030.57122237
GTGGTCG97000.030.36834742
GATCTCG109250.029.57814835
TGGTCGC104650.028.32192643
TCGGTGG109550.028.01689739
AGATCTC122750.026.39931934
TAGATCT132850.025.66200333
GTAGATC139800.025.57747832
GGTGGTC117100.025.26964841
AGTGTAG128250.024.82773229
CGGTGGT121350.024.56963740
GTCGTGT93250.022.94583314
AGCGTCG92650.022.89975211
GCGTCGT91800.022.8422712
GTGTAGA161000.022.48905230
GAGTGTA139000.022.29233628
Produced by FastQC (version 0.11.2)