HKHNTBCXY_l01n02_hk-90ar-2.35200000093fac.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKHNTBCXY_l01n02_hk-90ar-2.35200000093fac.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22445
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	677	3.016261973713522	Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGAA	116	0.5168188906215193	Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGGAAGAGCGTCGTGTA	113	0.5034528848295835	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA	101	0.4499888616618401	Illumina Single End PCR Primer 1 (100% over 27bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTAG	99	0.4410781911338828	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTGACTGGAGTTCAGACGTGTGCTCTTAGATCGGAAGAGCGTCGTGTAGGG	86	0.38315883270216083	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCGTCGTGTAGGGCAAGAGTGTAGATCTCGGTGGTCGCCG	85	0.3787034974381822	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGG	78	0.3475161505903319	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	60	0.26732011583871684	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGGA	60	0.26732011583871684	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCCG	55	0.24504343951882382	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	46	0.20494542214301625	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAG	45	0.20049008687903763	Illumina Single End PCR Primer 1 (100% over 28bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAAGATCGGAAGAGCGTCGTGT	34	0.15148139897527288	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGATCGGAAGAGCGCCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	29	0.12920472265537983	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	24	0.10692804633548675	Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	23	0.10247271107150813	Illumina Single End PCR Primer 1 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACT	40	6.315531E-9	45.0	33
ATGGGAG	20	6.8860356E-4	45.0	3
ACACGGT	20	6.8860356E-4	45.0	37
GGCACTT	20	6.8860356E-4	45.0	23
ACTCGGT	35	1.1422162E-7	45.0	37
TAGTGTA	40	6.315531E-9	45.0	28
GGGCACT	35	1.1422162E-7	45.0	22
GAGGAGG	45	3.4924597E-10	45.0	17
AACGAGT	20	6.8860356E-4	45.0	25
TCTGGAG	20	6.8860356E-4	45.0	4
GTCGAGT	20	6.8860356E-4	45.0	14
CACGAGT	45	3.4924597E-10	45.0	25
GTTCAGG	20	6.8860356E-4	45.0	12
TCGTGGA	35	1.1422162E-7	45.0	15
TGGAGCG	40	6.315531E-9	45.0	8
AGGGATA	20	6.8860356E-4	45.0	21
GAGCATC	35	1.1422162E-7	45.0	10
AGGTGGG	20	6.8860356E-4	45.0	1
AGGTGGC	35	1.1422162E-7	45.0	1
CAATAGT	20	6.8860356E-4	45.0	25

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content