FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_hk-90ar-1.35200000093f25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_hk-90ar-1.35200000093f25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516174
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG96910.275612071530021Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG53550.036.3125245
GGTCGCC52450.036.13006244
TGGTCGC52100.033.1324543
GTGGTCG52050.032.68818742
CTCGGTG54550.031.10493338
GATCTCG59850.030.79488835
ATCTCGG59700.030.570736
TCTCGGT56600.030.13737737
GGTGGTC57100.029.5967641
AGATCTC64150.029.15124134
TAGATCT70550.028.00554333
TCGGTGG61800.027.74680739
GTAGATC77100.027.72821432
CGGTGGT61450.027.72173940
CTCGTCG5350.026.91410811
TCGCGGT4350.026.90089637
GCGGCCG3950.026.2097545
TCTCGGG4750.026.05684337
GATCGCG5200.025.96610535
AGTGTAG70950.025.8815629
Produced by FastQC (version 0.11.2)