Basic Statistics
Measure | Value |
---|---|
Filename | HKHNTBCXY_l01n02_hk-60ar-2.35200000093f82.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 29453273 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1609430 | 5.464350260835188 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 166875 | 0.5665754023330446 | Illumina Single End PCR Primer 1 (100% over 51bp) |
AGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 55846 | 0.18960880850152037 | Illumina Single End PCR Primer 1 (98% over 51bp) |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 50987 | 0.17311149086894348 | Illumina Single End PCR Primer 1 (98% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGCCGT | 33865 | 0.0 | 39.401066 | 45 |
GTCGCCG | 274150 | 0.0 | 39.090355 | 45 |
GGTCGCC | 279645 | 0.0 | 38.250412 | 44 |
GTGGTCG | 281210 | 0.0 | 37.626427 | 42 |
TGGTCGC | 281285 | 0.0 | 37.57667 | 43 |
GGTGGTC | 284735 | 0.0 | 37.182743 | 41 |
CGGTGGT | 284390 | 0.0 | 36.87992 | 40 |
CTCGGTG | 289075 | 0.0 | 36.085396 | 38 |
TCGGTGG | 292065 | 0.0 | 35.814167 | 39 |
TCTCGGT | 291535 | 0.0 | 35.73638 | 37 |
TCGTGTG | 12850 | 0.0 | 34.214176 | 15 |
ATCTCGG | 308415 | 0.0 | 34.030697 | 36 |
GGTCGGA | 9440 | 0.0 | 31.619362 | 2 |
GTGACTG | 15535 | 0.0 | 31.101624 | 1 |
GATCTCG | 340095 | 0.0 | 30.77079 | 35 |
CGTGTGG | 12805 | 0.0 | 30.312067 | 16 |
TGACTGG | 16405 | 0.0 | 29.465536 | 2 |
GATCGAG | 10145 | 0.0 | 29.315823 | 8 |
AGGTCGG | 9815 | 0.0 | 29.21998 | 1 |
TCGCCGG | 3315 | 0.0 | 29.056133 | 45 |