FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_hk-60ar-2.35200000093f82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_hk-60ar-2.35200000093f82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29453273
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16094305.464350260835188Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1668750.5665754023330446Illumina Single End PCR Primer 1 (100% over 51bp)
AGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG558460.18960880850152037Illumina Single End PCR Primer 1 (98% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG509870.17311149086894348Illumina Single End PCR Primer 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT338650.039.40106645
GTCGCCG2741500.039.09035545
GGTCGCC2796450.038.25041244
GTGGTCG2812100.037.62642742
TGGTCGC2812850.037.5766743
GGTGGTC2847350.037.18274341
CGGTGGT2843900.036.8799240
CTCGGTG2890750.036.08539638
TCGGTGG2920650.035.81416739
TCTCGGT2915350.035.7363837
TCGTGTG128500.034.21417615
ATCTCGG3084150.034.03069736
GGTCGGA94400.031.6193622
GTGACTG155350.031.1016241
GATCTCG3400950.030.7707935
CGTGTGG128050.030.31206716
TGACTGG164050.029.4655362
GATCGAG101450.029.3158238
AGGTCGG98150.029.219981
TCGCCGG33150.029.05613345