FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_hk-60ar-1.35200000093f0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_hk-60ar-1.35200000093f0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804389
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC10000.033.7576144
GTCGCCG10400.033.10842545
ATCTCGG12650.028.8175336
CTCGGTG11700.028.65554238
GTGGTCG10650.028.10336942
TCTCGGT12600.026.43086837
GATCTCG13500.026.33708235
TGGTCGC11700.025.77432843
GGTGGTC13050.023.27788441
AGATCTC15500.023.22975534
TCGGTGG14450.023.20142239
GTAGATC18450.022.56481432
AGTGTAG15850.021.86491829
CGGTGGT13750.021.76492340
TAGATCT17400.021.598533
GATCGCG1056.103437E-821.43162235
CGTCGAG1656.002665E-1119.0904513
GTGTAGA21150.018.62018830
GCGGCCG1102.2525674E-618.41324245
GCCGACT2001.8189894E-1218.00056639
Produced by FastQC (version 0.11.2)