HKHNTBCXY_l01n02_hk-30ar-2.35200000093f68.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKHNTBCXY_l01n02_hk-30ar-2.35200000093f68.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5515982
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	685889	12.434576472512058	Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	70782	1.2832166602429087	Illumina Single End PCR Primer 1 (100% over 51bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCCG	42711	0.7743136217630877	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCGGAAGAGCGTCGTGTGGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	24089	0.43671281015782865	Illumina Single End PCR Primer 1 (98% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	20811	0.37728549513033216	Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	11947	0.21658881410417946	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	9138	0.16566406489361277	Illumina Single End PCR Primer 1 (100% over 41bp)
AGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	7083	0.12840868588766244	Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	6108	0.11073277614031374	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	5956	0.10797714713354756	Illumina Single End PCR Primer 1 (100% over 39bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	5881	0.1066174617683669	Illumina Single End PCR Primer 1 (100% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	13205	41.0723	45
GTGACTG	6705	39.85396	1
GTCGCCG	114860	39.48387	45
TGACTGG	6770	39.305187	2
TCGTGTG	4545	38.764946	15
GGTCGCC	117090	38.68384	44
GTGGTCG	117430	38.158424	42
GGTGGTC	117680	38.117702	41
TGGTCGC	117565	38.092674	43
CGGTGGT	117425	37.74754	40
AGATAGA	11320	37.339935	7
GACTGGA	7580	37.182587	3
GGTCGGA	3435	36.93228	2
CGTGTGG	4310	36.49347	16
GATCGAG	4190	36.401943	8
TCGCCGG	1050	36.22161	45
ACTGGAG	8175	36.072205	4
AGGTCGG	3470	35.263325	1
CTCGGTG	125665	35.055916	38
TCGGTGG	126040	35.043816	39