FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_hk-30ar-1.35200000093ee0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_hk-30ar-1.35200000093ee0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16776258
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG194100.11569922207920265Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC124150.036.29073344
GTCGCCG129900.035.94947445
GTGGTCG124650.031.10609442
CTCGGTG134450.030.29305538
ATCTCGG146350.029.90664936
TCTCGGT137700.029.34982337
GATCTCG151350.028.77005635
TGGTCGC140500.027.79000543
GGTGGTC140750.027.49851441
AGATCTC168300.026.24738934
TCGGTGG159750.026.00201839
TAGATCT189700.025.18385533
GTAGATC203250.024.82252332
CGGTGGT155800.024.78413840
CTCGGGG14450.024.76022338
AGTGTAG179150.023.28780629
GGGTCGC20650.023.10368543
GGCCGCC19350.021.86529244
GTGTAGA229900.021.80793230
TGTAGAT233250.021.42725231
Produced by FastQC (version 0.11.2)