FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_hk-120ar-2.35200000093fc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_hk-120ar-2.35200000093fc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35724443
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG500623314.013466913955803Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT5600401.5676661494764241Illumina Single End PCR Primer 1 (100% over 51bp)
AGATCGGAAGAGCGTCGTGTGGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1995940.5587043022616196Illumina Single End PCR Primer 1 (98% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1822510.5101577091068992Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1029650.2882200290708521Illumina Single End PCR Primer 1 (98% over 51bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG730790.20456302145844515Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG647480.18124285380740576Illumina Single End PCR Primer 1 (100% over 41bp)
AGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG607820.17014121115898154Illumina Single End PCR Primer 1 (98% over 51bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC571580.15999689624272098Illumina Single End PCR Primer 1 (100% over 50bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT537300.15040122528992264Illumina Single End PCR Primer 1 (100% over 47bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC504710.1412786197954157Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT489180.1369314561461462Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT1061000.041.71392445
TCGTGTG377000.039.67733415
GTCGCCG8629500.039.03956645
GTGACTG394500.038.8351331
GGTCGCC8836950.038.02789344
TCGCCGG85000.038.02033245
GGTCGGA285650.037.8048482
TGACTGG406450.037.631522
TGGTCGC8884950.037.51460643
GTGGTCG8898150.037.5029742
GGTGGTC8925300.037.40425541
CGGTGGT8892900.037.13489540
AGGTCGG283400.036.796331
CGTGTGG364350.036.7399116
AGATAGA703100.036.582937
GATCTGA396150.036.2081832
GATCGAG282700.035.9615368
GACTGGA454350.035.2445833
CTCGGTG9378050.035.01926438
TCGGTGG9419900.034.9660139
Produced by FastQC (version 0.11.2)