FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_hk-120ar-1.35200000093f5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_hk-120ar-1.35200000093f5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474803
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG77060.311378319809698Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG43900.037.42355345
GGTCGCC42800.036.8078944
CTCGGGG3550.035.49429738
GTGGTCG40350.034.0202842
TGGTCGC41550.033.47170343
GGGTCGC5050.032.97620843
CTCGGTG43800.032.31286238
ATCTCGG47950.032.00393736
TCTCGGT45400.031.52101137
CGTCGAG4500.031.4986413
GATCTCG48850.031.36824435
GGTGGTC44100.031.07448641
TCGGTGG46950.030.72000939
AGATCTC50400.030.62863234
TCTCGGG4000.030.37614437
CGGTGGT44700.030.2528240
TAGATCT55700.029.97722833
GTAGATC60450.029.48332
GGCCGCC4600.029.35486644
CTCGTCG3950.029.0493811
Produced by FastQC (version 0.11.2)