FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_h-30ar-2.35200000093ffc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_h-30ar-2.35200000093ffc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12938340
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG310740.24016991360560935Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC152550.035.6282244
GTCGCCG158800.035.5585845
TGGTCGC152250.032.74051343
GTGGTCG152250.032.57653442
GAGCGGT40300.028.97839216
GGTGGTC174450.028.19732741
GGCCGCC24450.028.16642844
CTCGTCG21150.027.44516811
GCCGCCG27000.027.00694545
CGGTGGT188200.026.54363640
CTCGGGG14050.025.625638
TCGCCGT34900.025.34319545
CGGTTCA46000.025.04449819
CGTCGAG22700.024.87794313
AGAGCGG65850.024.63596715
TGCCGAG26800.024.18345633
GCGGCCG17150.024.01492345
AATGCCG31100.023.95116231
GCCGAGA27600.023.48275834
GATCGCG21200.023.24647735
Produced by FastQC (version 0.11.2)