FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n02_h-30ar-1.35200000093fd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n02_h-30ar-1.35200000093fd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13793708
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG689830.5001048304052833Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT148610.10773752786415372Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG312650.036.37294845
GGTCGCC304250.035.9417544
TGGTCGC285650.033.82969743
GTGGTCG284500.033.61717642
GGCCGCC39850.033.4895544
GGGTCGC36750.032.2718343
GAGCGGT68450.032.18202616
GCCGCCG44100.031.58927745
CTCGGGG33450.031.48333238
CGGCGTG34650.031.42945519
GGTGGTC304350.031.23796841
GCGGCCG27300.030.83163845
TCGCCGT59350.030.82886145
AGAGCGG115650.030.38940815
CTCGTCG36250.030.34971611
CAGATCG188800.030.202746
CGGTTCA72600.030.09382219
TCTCGGG37900.029.68679637
CGGTGGT327950.029.68303340
CGTCGAG37450.029.25771313
Produced by FastQC (version 0.11.2)