FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_k-30ar-1.35100000094024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_k-30ar-1.35100000094024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14192883
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC335570.2364354021660011TruSeq Adapter, Index 13 (98% over 50bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG151690.10687751036910541Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT77150.031.1751910
AGAGCGG79800.030.1681169
GCGGTTC83700.028.84307712
AAGAGCG86400.028.1760988
AGCGGTT89750.026.9489211
ATGCCGA46900.026.2416426
CGGTTCA93350.025.88554213
CCGAGAG29100.025.51546929
CAGCAGG93650.025.51432218
AATGCCG56550.025.46338825
GCCGAGA44750.025.2401428
CTCGTAT81950.025.06791543
GTCACAG76350.025.04912430
AGCAGGA94600.025.0440519
TGCCGAG46550.025.03751227
GGAATGC77550.024.9508623
CAGTCAC84600.024.94672828
CGTATGC82800.024.9459245
TCCAGTC88450.024.42023726
GAACTCC91550.024.35477622
Produced by FastQC (version 0.11.2)