FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-90ar-2.35100000093faf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-90ar-2.35100000093faf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22445
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC317914.163510804188014TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCAC12285.471151704165739TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGATCGGAAGAGCAC7683.421697482735576TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGATCGGAAGAGCAC4642.0672755624860772TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGATCGGAAGAGCAC3231.4390732902650925TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGATCGGAAGAGCAC2631.1717531744263756TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATCGGAAGAGCAC1880.8376030296279795TruSeq Adapter, Index 25 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT1580.7039429717086211TruSeq Adapter, Index 14 (97% over 35bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT1560.6950323011806638TruSeq Adapter, Index 14 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTG1540.6861216306527067TruSeq Adapter, Index 14 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCACA1450.6460236132768991TruSeq Adapter, Index 5 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC1440.6415682780129205TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGATCGGAAGAGCAC1380.6148362664290488TruSeq Adapter, Index 3 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA1120.4989975495656048TruSeq Adapter, Index 14 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA1070.4767208732457118TruSeq Adapter, Index 14 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACAC910.40543550902205394TruSeq Adapter, Index 13 (97% over 35bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC850.3787034974381822TruSeq Adapter, Index 14 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATCGGAAGAGCAC830.369792826910225TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGATCGGAAGAGCACA800.35642682111828916TruSeq Adapter, Index 3 (97% over 37bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC760.3386054800623747Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGATCGGAAGAGCACA750.3341501447983961TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATCGGAAGAGCAC720.32078413900646024TruSeq Adapter, Index 3 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCACACGTCT650.28959679215860995TruSeq Adapter, Index 5 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG510.22722209846290933TruSeq Adapter, Index 14 (97% over 38bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC440.19603475161505904TruSeq Adapter, Index 14 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACAC420.1871240810871018TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGATCGGAAGAGCAC380.16930274003118734TruSeq Adapter, Index 3 (97% over 37bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG370.16484740476720872TruSeq Adapter, Index 14 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGATCGGAAGAGCACA350.1559367342392515TruSeq Adapter, Index 3 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCAGATCGGAAGAGCACACGTCT350.1559367342392515TruSeq Adapter, Index 3 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGATCGGAAGAGCAC310.13811539318333704TruSeq Adapter, Index 2 (97% over 36bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG310.13811539318333704TruSeq Adapter, Index 13 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGATCGGAAGAGCAC310.13811539318333704TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGATCGGAAGAGCAC300.13366005791935842TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGATCGGAAGAGCACA290.12920472265537983TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGATCGGAAGAGCACA230.10247271107150813TruSeq Adapter, Index 3 (97% over 37bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCA230.10247271107150813Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAG206.8860356E-445.040
TGACTGG206.8860356E-445.02
ATAGGAA206.8860356E-445.039
GTGACTG206.8860356E-445.01
GACTGGA206.8860356E-445.03
ACTGGAG206.8860356E-445.04
GTCACAT253.769293E-544.99999630
CACATAG253.769293E-544.99999632
ACATAGA253.769293E-544.99999633
ACAGAGA555.4569682E-1140.9090933
ACTTAGA555.4569682E-1140.9090933
CACTTAG555.4569682E-1140.9090932
CACAGAG555.4569682E-1140.9090932
GTCACTT601.382432E-1037.49999630
AGGAAGA301.10459136E-437.49999641
TGGAGTT301.10459136E-437.4999966
GTTCAGA301.10459136E-437.49999610
GAGTTCA301.10459136E-437.4999968
CAGAGAT601.382432E-1037.49999634
AGACGTG301.10459136E-437.49999614
Produced by FastQC (version 0.11.2)