FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-90ar-1.35100000093f28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-90ar-1.35100000093f28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516174
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC227330.6465265939626423TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG104460.29708427398644094Illumina Paired End PCR Primer 2 (97% over 34bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC52690.14985037714288313Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT42450.034.9278810
ATGCCGA23550.034.39446326
GCGGTTC43500.034.1365212
TATCTCG31500.033.8586340
AGCGGTT44100.033.67207711
AGAGCGG44650.033.307699
CTCGTAT36750.033.30758743
AATGCCG28900.033.00948325
AAGAGCG45400.032.7574548
CAGCAGG44300.032.60587718
ATCTCGT35900.032.34125541
GGAATGC37350.032.2275823
CCGAGAG15900.031.98071929
GGTTCAG46500.031.88577714
CGGTTCA46900.031.661813
AGCAGGA45450.031.63235519
TGCCGAG24800.031.02782227
GAATGCC38250.030.41093824
AGGAATG41250.030.3805622
CGTATGC40800.030.11033245
Produced by FastQC (version 0.11.2)