FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-60ar-2.35100000093f85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-60ar-2.35100000093f85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29453273
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC19183296.513126741466051TruSeq Adapter, Index 16 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC2283700.775363743105902TruSeq Adapter, Index 16 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC917880.3116393889398981TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2665500.037.5000845
TCGTATG2738400.036.44658344
CATCTCG2606500.036.35825740
ATCTCGT2645900.036.2182341
CTCGTAT2740050.036.20866443
TCTCGTA2657550.036.0686542
GTCCATC2684300.034.3824837
CCATCTC2746500.034.0469739
TCCATCT2755250.033.69104838
GTATGCC441100.033.47207345
CGTCCAT2812850.032.69352336
TCACGCG29250.031.4615531
CCGTCCA3227900.029.32201835
AGTCACG71500.028.29017329
GTCACGC37450.027.93726230
CCCGTCC3423550.027.6594634
CACGCGT25600.026.45562732
ACCCGTC3631950.026.2240633
CACCCGT3710100.025.78690332
TCACCCG3765750.025.57678831
Produced by FastQC (version 0.11.2)