FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-60ar-1.35100000093f0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-60ar-1.35100000093f0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804389
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC32620.18078141686742713TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG20400.11305766106975824Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT10200.030.88169110
AGAGCGG10350.029.9993539
AGCGGTT10550.029.6439111
GCGGTTC10500.029.57079112
AAGAGCG10850.029.0316358
ATGCCGA6350.027.99152426
GGAATGC9350.027.43256623
AATGCCG7300.027.43091425
TATCTCG6350.027.2843940
CCGAGAG3300.027.27289629
CAGCAGG12000.026.24943418
CGGTTCA12450.026.02353713
GGTTCAG12250.025.897414
AGCAGGA12000.025.31195419
GTTCAGC12450.024.75850315
CTCGTAT7300.024.65837143
AGGAATG11150.024.01293622
TGCCGAG6500.023.53795227
CAGGAAT12450.023.13203221
GAATGCC11100.023.10760924
Produced by FastQC (version 0.11.2)