FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-30ar-2.35100000093f6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-30ar-2.35100000093f6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515982
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC79237214.365021495719166TruSeq Adapter, Index 14 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC960361.7410499164065438TruSeq Adapter, Index 14 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC616321.117335045690867TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAACTCGTATGC103970.18848864989044561TruSeq Adapter, Index 14 (97% over 39bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA103810.18819858367920708TruSeq Adapter, Index 14 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTC101470.1839563653398434TruSeq Adapter, Index 14 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCACCTCGTATGC81950.14856828756874116TruSeq Adapter, Index 14 (97% over 39bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG77400.1403195296866451TruSeq Adapter, Index 14 (97% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC69520.12603376878314687TruSeq Adapter, Index 14 (100% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG65860.1193985042010652TruSeq Adapter, Index 14 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1093000.037.4969245
TCGTATG1118550.036.58743344
ATCTCGT1084300.036.26178741
TCTCGTA1084700.036.23771342
CTCGTAT1123900.036.1890443
CATCTCG1079850.036.10907740
AGTCACG20000.033.9753229
GTATGCC182400.033.86134345
GTCACGG12650.033.6169230
CCATCTC1165100.033.11356439
TCCATCT1166500.032.9451838
TTCCATC1162050.032.5124937
GTTCCAT1219650.031.01023736
AGTTCGG12700.029.9507311
GTTCGGA13100.029.3705062
AGTTCCA1420650.027.37984335
CACCTCG16150.027.1686740
CAGTTCC1514300.025.70886234
ACAGGTC11900.025.52614633
CCTCGTA18100.025.23596242
Produced by FastQC (version 0.11.2)