FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-30ar-1.35100000093ee3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-30ar-1.35100000093ee3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16776258
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC341850.20377011369281517TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG264990.15795536763919582Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT123350.032.04799310
AGAGCGG127950.031.0716639
GCGGTTC130550.030.3494412
AAGAGCG132500.030.0386338
ATGCCGA74000.029.61433226
AGCGGTT134600.029.41953711
AATGCCG87700.029.19524825
CAGCAGG136750.028.46341518
GGAATGC117250.027.9393823
CCGAGAG48300.027.76372529
GATCTCG58950.027.71066340
AGCAGGA140150.027.6444719
CGGTTCA148450.026.78086313
GGTTCAG148450.026.53836414
AGGAATG129100.026.35079822
CAGGAAT143500.025.79183421
CGAGAGA54500.025.10068930
GAATGCC125850.024.95746224
CTCGTAT75200.024.83414343
CGTATGC81050.023.62430245
Produced by FastQC (version 0.11.2)