FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-120ar-2.35100000093fc8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-120ar-2.35100000093fc8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35724443
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC627186817.556237335876727TruSeq Adapter, Index 18 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC7863562.2011707782259893TruSeq Adapter, Index 18 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC2344360.656234164378714TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCAACTCGTATGC1063450.29768133823668014TruSeq Adapter, Index 18 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACCTCGTATGC806910.22587056150882465TruSeq Adapter, Index 18 (98% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG793050.2219908649100561TruSeq Adapter, Index 18 (100% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA764120.213892768041198TruSeq Adapter, Index 18 (100% over 40bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG619290.1733518980267936TruSeq Adapter, Index 18 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG586380.16413971800763977TruSeq Adapter, Index 18 (100% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCCGTC571780.16005288032062528TruSeq Adapter, Index 18 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCAGCTCGTATGC472480.13225678564113652TruSeq Adapter, Index 18 (98% over 50bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGC374460.1048189890602353TruSeq Adapter, Index 18 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8420550.037.86208745
TCGTATG8653350.036.7802444
ATCTCGT8330500.036.52438441
TCTCGTA8331450.036.5169842
GTATGCC1331400.036.5151745
CTCGTAT8707000.036.42786443
CATCTCG8247400.036.4105940
GCATCTC8738000.034.07634739
CGCATCT8702850.033.98690438
CCGCATC8738000.033.7107337
AGTTCGG84200.033.012091
TCCGCAT9120150.032.342636
GTTCGGA87300.032.2431762
ACGTGCG45600.031.77689633
TCACGTG125950.031.03030431
CACGTGC68650.031.00583832
CGTGCGC36900.030.97643534
GTCCGCA10444650.029.41548535
CACCTCG147500.029.28932840
CCTACAC180550.028.61149411
Produced by FastQC (version 0.11.2)