FastQCFastQC Report
Sat 8 Jul 2017
HKHNTBCXY_l01n01_hk-120ar-1.35100000093f5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKHNTBCXY_l01n01_hk-120ar-1.35100000093f5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474803
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC164210.6635275615877304TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG104950.4240741586300001Illumina Paired End PCR Primer 2 (97% over 34bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC54330.21953262542513483Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA24950.035.2626
GAGCGGT42100.034.95197710
GCGGTTC42450.034.55779312
AATGCCG29550.034.41573325
TGCCGAG24150.034.37837227
AGAGCGG43150.034.153619
AGCGGTT43750.033.6337911
CTCGTAT26350.033.47266843
AAGAGCG44250.033.3045928
CCGAGAG15850.033.21717529
AGCAGGA43500.033.05123519
CAGCAGG43650.033.0407518
CGGTTCA44500.032.8141213
GGAATGC38050.032.7590423
GGTTCAG44700.032.61696614
ATCTCGT25400.032.42143641
GAATGCC36250.032.21331424
AGGAATG40300.032.102522
GCCGAGA25450.032.0918628
CAGGAAT43250.031.889721
Produced by FastQC (version 0.11.2)